Purification and Characterization of Glycyrrhetic Acid Mono-glucuronide .BETA.-D-Glucuronidase in Eubacterium sp. GLH.
نویسندگان
چکیده
منابع مشابه
Simultaneous determination of glycyrrhizin, glycyrrhetic acid and glycyrrhetic acid mono-glucuronide in Shakuyaku-kanzo-to incubated with rat feces by semi-micro high-performance liquid chromatography.
A method for semi-micro high-performance liquid chromatography (HPLC) has been established for the simultaneous determination of glycyrrhizin (GL), glycyrrhetic acid (GA) and glycyrrhetic acid mono-glucuronide (GAMG) in incubation mixtures of rat feces with Shakuyaku-kanzo-to decoction (combination of licorice root and peony root). The analysis could be accomplished within 20 min with a TSKgel ...
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In recent years, interest in the metabolism of glucuronic acid has grown. Contributions have ranged from studies of its biosynthesis and conjugation in surviving tissues and exper,imental animals to its utilization in man (l-lo). In all spheres, especially the latter, progress has been hampered by the lack of an analytical method by which glucuronides could be differentiated from free glucuroni...
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In 1960, Linker, Meyer, and Hoffman (1) reported that a crude leech preparation hydrolyzes the glucuronide linkages of hyaluronic acid, unlike bacterial and testes hyaluronidases, which act on glucosaminide linkages. This paper deals with the first systematic attempt to purify the leech P-glucuronidase that specifically hydrolyzes hyaluronic acid. Purified preparations of the enzyme have indeed...
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Gene-mediated enzyme prodrug therapy (GDEPT) seeks to increase the therapeutic index of anti-neoplastic agents by promoting selective activation of relatively nontoxic drug derivatives at sites of specific enzyme expression. Glucuronide prodrugs are attractive for GDEPT due to their low toxicity, bystander effect in the interstitial tumor space and the large range of possible glucuronide drug t...
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P-Glucuronidase has been purified from mouse kidneys previously induced by gonadotrophin to a specific enzyme activity 15 times higher than the non-induced kidney. The purification procedure includes ultrasonication to solubilize the enzyme, acid and ammonium sulfate precipitations, gel filtration in Sephadex G-200, DEAE-ion exchange chromatography, and isoelectric focusing. The resulting produ...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 1999
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.22.80